An ornithine decarboxylase-deficient mutant of Chinese hamster ovary cells.
نویسندگان
چکیده
منابع مشابه
Expression of human chromosome 2 ornithine decarboxylase gene in ornithine decarboxylase-deficient Chinese hamster ovary cells.
Ornithine decarboxylase (ODC) belongs to a multigene family and some of these may very well be nonfunctional (pseudogenes). We isolated an ODC gene from a human chromosome 2-specific library and transfected the gene into ODC-deficient Chinese hamster ovary cells to directly demonstrate that this ODC gene is functional and ODC is essential for cell proliferation. After screening 2.5 X 10(5) plaq...
متن کاملExpression of Human Chromosome 2 Ornithine Decarboxylase Gene in Ornithine Decarboxylase-deficient Chinese Hamster Ovary Cells1
Ornithine decarboxylase (OIK ) belongs to a multigene family and some of these may very well be nonfunctional (pseudogenes). We isolated an ODC gene from a human chromosome 2-specific library and transfected the gene into ODC-deficient Chinese hamster ovary cells to directly demonstrate that this ODC gene is functional and OIK is essential for cell proliferation. After screening 2.5 x 10* plaqu...
متن کاملSqualene synthase-deficient mutant of Chinese hamster ovary cells.
Squalene synthase (farnesyldiphosphate:farnesyldiphosphate farnesyltransferase, EC 2.5.1.21) converts farnesyl pyrophosphate to squalene, the first metabolic step committed solely to the biosynthesis of sterols. Using a fluorescence-activated cell sorting technique designed to screen for cells defective in the regulated degradation of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, w...
متن کاملRetinol inhibition of ornithine decarboxylase induction and G1 progression in Chinese hamster ovary cells.
sequential events of the growth response under controlled conditions without the influence of a transforming or promoting stimulus. We have previously demonstrated in mitotically syn chronized CHO4cells that vitamin A treatment arrested cells in mid-G1 phase (16, 17). The inhibitory effect of vitamin A was a function of cell cycle position; cell cycle progression was blocked only when retinol w...
متن کاملMolecular and genetic characterization of an ornithine decarboxylase-deficient Chinese hamster cell line.
The ornithine decarboxylase (ODC)-deficient Chinese hamster ovary (CHO) cell line C55.7 has normal amounts of ODC mRNA with very low amounts of immunologically detectable ODC protein, suggesting a structural mutation; however, 5-azacytidine treatment leads to phenotypical reversion (Steglich, C., and Scheffler, I. E. (1985) Somat. Cell Mol. Genet. 11, 11-23). We have demonstrated by chemical cl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34767-7